
So we are back in Guayquil, this time for sample collection. We are staying at the Kennedy Hotel in downtown Guayaquil and it is apartment living with our own living area, kitchen, and bathroom. On Saturday, we collected samples from our first site in the Guayas province, Santo Domingo. We have met up with three more members of our research team: Professor Carlos Vasquez, Jefferson Ordonez, Yarixa Candicuz. All of them are from Colegio Tecnico Agropecuario Jose Otilio Ramirez, San Lorenzo, in te province of Esmeraldas, Ecuador. Esmeraldas will be our last province on our journey. Carlos is extremely knowledgable and was able to help me in identifying all of the different mangrove species and taxonomically classifying them by examing their leaves, roots, and flowers. I feel pretty confident in distinguishing between what is rhizophora mangle in comparison to conocarpus erectus, whereas rhizophora harrosonii is more difficult to tell apart from rhizophora mangle. We collected from only one site because the tide was changing and we had to move on. However, we were able to find 5 different species in one site which was remarkable for our first try. I'm beginning to wonder how many of these plant species are natural as opposed to replanted.
Because Saturday was such a stressful day on the water, Acacia decided to give us Sunday off to watch the World Cup Final and relax. Joe, Shana, Brenna, Diana, and I all went to Didi's cousin's restaurant in downtown Guayquil to eat and watch the game. Spain won which was good because I'm pretty sure Joe was the only one in the entire restaurant rooting for Netherlands. Last night we all went out to Lo Nuestro for Daniela's birthday. It was a lot of fun and we ended dinner with medley of spanish songs sung by the majority of the table. This singing led us in search of a place for karaoke but because it was Sunday, everything was closed. Some of us walked around Las Penas and el malecon which is the equivalent to a boardwalk in the US. It was really relaxing and great getting to know everyone on a more personal level.
This morning, I had to get up bright and early for our third lecture in the series. It was at the central government building in Guayaquil. It was difficult to get in because there was tight security and so many people were present. At one point, I heard someone was yelling at Diana because we had to limit the number of students/participants who could attend the talk. Brenna and everyone else is still there now while I'm trying to clean up the apartment. My spanish is improving which is a plus but it's difficult because the majority of people here want to learn english from Brenna and I. I find myself repeating words like "breakfast" or "fast" in english to a majority of the team here.
Also, I have been updating my database of samples and my daily report. For example here is the entry of a typical research day for me:
Saturday, July 10, 2010- Santo Domingo/ Taura, Guayaquil
Team: Ben, Brenna, Joe, Shana, Gober, Carlos, Jefferson, Yarixa, Daniela, Sofia, Miriam, Oscar
Mangrove leave/roots: #26-32
Sediment: #11-12
Water: #27-30
On our third day of field work, we went to Santa Domingo. We collected mangrove leaves, roots, sediment and water samples from 1 site due to time constraints and the changing tide. The first site was located where an estuary met the open ocean. In site 1, we collected rhizophora mangle, rhizophora harrisonii, avicennia germinans, conocorpus erectus, and laguncularia racemosa samples. The sediment collected was taken near the roots of the mangrove trees at the site and placed in large Ziploc bags. We took one sediment sample at each site (2 replicates of each). Water samples were not taken because that morning information was given that centrifuge tubes would not be enough for analysis. Alternatives for water collected are being sought. Leaves were placed in plastic Ziploc bags (3 replicates of each sample taken) for later pressing and taxononomic classification. The root tip tissues (about 1g) of each mangrove sample were placed in 5mL of RNAlater and 5mL of ethanol for RNA and DNA analysis respectively. The rest of the root tip was placed in a small Ziploc bag for pollutant analysis (heavy metals and POPs) and immediately placed on ice. The collection was from about 12:30am – 2:30pm.
-Ben
